Keywords
onion extract
sugar paste
mixture
Staphylococcus aureus
Escherichia coli
How to Cite
Abstract
Background: Staphylococcus aureus and Escherichia coli are infection agents. The onion is known to have antibacterial properties. Meanwhile, sugar paste is effective to inhibit bacterial growth.
Objective: This study aimed to identify the antibacterial activity of onion extract (Allium cepa Linn) and sugar paste mixture on staphylococcus aureus and Escherichia coli by in vitro.
Methods: We used a post-test-only control group design with a completely random design. The onion extract with sugar paste mixture was divided into 4 concentrations; 25%, 50%, 75% and 100% with 3 repetitions. The data were analyzed by measuring inhibition zone diameter and tested using the One-Way Analysis of Variance (ANOVA) and Tukey test.
Results: Findings showed that on average, the antibacterial activity of onion extract and sugar paste mixture on staphylococcus aureus was 14.57mm, 17.44mm, 18.36mm, and 22.28mm, respectively 25%, 50%, 75%, and 100% concentrations. Meanwhile, on Escherichia coli, it was 17.27mm, 19.67mm, 20.31mm, and 21.62mm.
Conclusion: onion extract and sugar paste mixture can inhibit the growth of Staphylococcus aureus and Escherichia coli.
References
A. G. Tanner, E. R. T. C. Owen, D. V. Seal. 1988. "Successful Treatment of Chronically Infected Wounds with Sugar Paste.” European Journal of Clinical Microbiology and Infectious Diseases 7(4): 524–525.
Agra, Isabela K R et al. 2013. "Evaluation of Wound Healing and Antimicrobial Properties of Aqueous Extract from Bowdichia Virgilioides Stem Barks in Mice.” Anais da Academia Brasileira de Ciencias 85(3): 945–54.
Archer, Helen G et al. 1990. "A Controlled Model of Moist Wound Healing: Comparison between Semi-Permeable Film , Antiseptics and Sugar Paste.” J. Exp. Path. 71: 155–70.
Bakht, Jehan, Shehla Khan, and Mohammad Shafi. 2013. "Antimicrobial Potentials Of Fresh Allium Cepa Against Gram Positive And Gram Negative Bacteria And Fungi.” Pak. J. Bot. 45(1): 1–6.
Bhat, Dr Rahul R et al. 2014. "Comparison of Sugar and Honey Dressings in Healing of ChronicnWoundsn.” IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) 13(5): 82–88. http://www.iosrjournals.org/iosr-jdms/papers/Vol13-issue5/Version-3/T013538288.pdf.
Biswas, Atanu et al. 2010. "Use of Sugar on the Healing of Diabetic Ulcers: A Review.” Journal of Diabetes Science and Technology 4(5): 1139–45.
Bradshaw, Claire E, Howard Street, and S Sheffield. 2011. "An in Vitro Comparison of the Antimicrobial Activity of Honey, Iodine and Silver Wound Dressings.” Bioscience Horizons 4(1): 61–70.
Chanprapaph, Kumutnart et al. 2012. "Effectiveness of Onion Extract Gel on Surgical Scars in Asians.” Hindawi Publishing Corporation 2012: 1–6.
Connell, Kristen O, Jennifer L Wardlaw, and Diplomate Acvs. 2011. "Unique Therapies for Difficult Wounds.” Today's Veterinary Practice (August): 10–16.
Joaheer D. Teshika, Aumeeruddy M. Zakariyyah et al. 2019. "Traditional and Modern Uses of Onion Bulb (Allium Cepa L.): A Systematic Review.” Critical Reviews in Food Science and Nutrition 59.
Mathews, Karol A, and Allen G Binnington. 2002. "Wound Management Using Sugar.” Compend Contin Educ Pract Vet 24(1): 41–50.
Misna, and Khusnul Diana. 2016. "Aktivitas Antibakteri Ekstrak Kulit Bawang Merah (Allium Cepa L.) Terhadap Bakteri Staphylococcus Aureus.” Galenika Journal of Pharmacy 2(2): 138–44.
Molan, Peter, and Tanya Rhodes. 2015. "Honey: A Biologic Wound Dressing.” WOUNDS 27(6): 141–51.
Murandu M, Webber MA, Simms MH, Dealey C. 2011. "Use of Granulated Sugar Therapy in the Management of Sloughy or Necrotic Wounds: A Pilot Study.” J Wound Care 20(5): 206.
Naselli, Aldo et al. 2017. "Granulated Sugar for Adjuvant Treatment of Surgical Wound Infection due to Multi-Drug-Resistant Pathogens in a Child with Sarcoma: A Case Report and Literature Review.” Le Infezioni in Medicina (4): 358–61.
Nile, Shivraj Hariram, and Se Won Park. 2013. "Total Phenolics, Antioxidant and Xanthine Oxidase Inhibitory Activity of Three Colored Onions (Allium Cepa L.).” Frontiers in Life Science 7(Nos. 3–4): 224–228.
Nor, Theresia Avilla, Desi Indriarini, Sangguana Marten, and Jacobus Koamesah. 2018. "Uji Aktivitas Antibakteri Ekstrak Etanol Daun Pepaya (Carica Papaya L) Terhadap Pertumbuhan Bakteri Escherichia Coli Secara In Vitro.” Cendana Medical Journal 15(5): 327–37.
Octaviani, Melzi, Haiyul Fadhli, and Erenda Yuneistya. 2019a. "Uji Aktivitas Antimikroba Ekstrak Etanol Dari Kulit Bawang Merah ( Allium Cepa L .) Dengan Metode Difusi Cakram Antimicrobial Activity of Ethanol Extract of Shallot ( Allium Cepa L .) Peels Using the Disc Diffusion Method.” Pharmaceutical Sciences and Research (PSR) 6(1): 62–68.
Praba, S Keerthi, and R Kumaresan. 2014. "Efficacy of Antimicrobial Activity of Aqueous Garlic (Allium Sativum) Extract against Different Bacterial Species.” Journal of Chemical and Pharmaceutical Research 6(10): 677–79.
Razak, Abdul, Aziz Djamal, and Gusti Revilla. 2013. "Artikel Penelitian Uji Daya Hambat Air Perasan Buah Jeruk Nipis ( Citrus Aurantifolia S .) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara In Vitro.” Jurnal Kesehatan Andalas 2(1): 5–8. http://jurnal.fk.unand.ac.id/articles/vol_2no_1/05-08.pdf.
Ruhullah, Mohammad et al. 2013. "Experience with the Use of Sugar Paste Dressing Followed by Reconstruction of Sacral Pressure Sore with V-Y Flap: A Reliable Solution for a Major Problem.” academic Journls 4(4): 27–32.
Sharifi-Rad, J. et al. 2016. "Plants of the Genus Allium as Antibacterial Agents: From Tradition to Pharmacy.” Cell. Mol. Biol 62(9): 57–68.
Shenoy, Chitra, M B Patil, Ravi Kumar, and Swati Patil. 2009. "Preliminary Phytochemical Investigation and Wound Healing Activity of.” International Journal of Pharmacy and Pharmaceutical Sciences 2(2): 167–75.
Sidgwick, G. P., D. McGeorge, and A. Bayat. 2015. "A Comprehensive Evidence-Based Review on the Role of Topicals and Dressings in the Management of Skin Scarring.” Archives of Dermatological Research 307(6): 461–77.
Simaremare, Ade Pryta R. 2017. "Perbedaan Aktivitas Antibakteri Ekstrak Tanaman Bawang Merah (Allium Cepa L) Dan Bawang Putih ( Allium Sativum L) Pada Berbagai Konsentrasi Terhadap Bakteri Staphylococcus Aureus In Vitro.” Nommensen Journal of Medicine 3(2): 52–27.
Å kerget, M, L Majheniè, and M Bezjak. 2009. "Antioxidant , Radical Scavenging and Antimicrobial Activities of Red Onion ( Allium Cepa L ) Skin and Edible Part Extracts.” Chem. Biochem. Eng. Q. 23(4): 435–44.
Surono, Angela Stevy. 2013. "Antibakteri Ekstrak Etanol Umbi Lapis Bawang Merah (Allium Cepa L.) Terhadap Pertumbuhan Staphylococcus Aureus Dan Escherichia Coli.” Calyptra: Jurnal Ilmiah Mahasiswa Universitas Surabaya 2(1): 1–15.
Topham, J. 2002. "Why Do Some Cavity Wounds Treated with Honey or Sugar Paste Heal without Scarring ?” Woundcare Journal 11(2): 1–5.
Wang, Yi, Wei-xi Tian, and Xiao-feng Ma. 2012. "Inhibitory Effects of Onion ( Allium Cepa L .) Extract on Proliferation of Cancer Cells and Adipocytes via Inhibiting Fatty Acid Synthase.” Asian Pacific J Cancer Prev 13(11): 5573–79.
Yunanda, V, and T Rinanda. 2017. "Aktivitas Penyembuhan Luka Sediaan Topikal Ekstrak Bawang Merah (Allium Cepa) Terhadap Luka Sayat Kulit Mencit (Mus Musculus). Jurnal Veteriner 17(4): 606–14. http://ojs.unud.ac.id/index.php/jvet/article/view/26568/16883.
Zaenal, Suryani As'ad, Veni Hadju, and Agussalim Bukhari. 2016. "Role of Topical Cream Extract Garlic (Allium Sativum) in Wound Healing Fibroblasts Judging from the Strain Wistar Rats with Acute Injury Models.” International Journal of Sciences: Basic and Applied Research (IJSBAR) 26(3): 35–47. http://gssrr.org/index.php?journal=JournalOfBasicAndApplied.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright (c) 2019 Tahiruddin Tahiruddin, diah indriastuti