A NOVEL STRAIN OF SHIGELLA SPECIES OUTBREAK IN A RESIDENTIAL SCHOOL IN PEMAGATSHEL, BHUTAN, 2012
PDF

Supplementary Files

A novel strain of Shigella species outbreak in a residential school in Pemagatshel, Bhutan, 2012

Keywords

Shigellosis
MDR-Shigella
Boarding school
Bhutan

How to Cite

Tsheten, T., Tshering, D., Gyem, K., Dorji, S., Wangchuk, S., Irfani, T. H., & Letho, L. (2016). A NOVEL STRAIN OF SHIGELLA SPECIES OUTBREAK IN A RESIDENTIAL SCHOOL IN PEMAGATSHEL, BHUTAN, 2012. Public Health of Indonesia, 2(4), 165–171. https://doi.org/10.36685/phi.v2i4.98

Abstract

Background: A cluster of suspected shigellosis was reported from health center in Pemagatshel district to Royal Center for Disease Control on 14th May 2012. The investigation was done to determine the cause and risk factor for the outbreak so that appropriate control and prevention measures can be implemented.

Methods: A descriptive study was used for the outbreak investigation. The food items and drinks served to boarding students were collected from the mess in-charge in order to assess their risk for the outbreak. The kitchen and its premises were inspected to study the likely contamination by rodents and other animals. The water and stool specimens were tested in the laboratory to identify all possible enteric pathogens.

Results: 82 boarding students were affected with an overall attack rate of 28% (82/294). Diarrhea was the predominant symptom followed by abdominal pain and headache. The onset date of the cases varied between 11th and 18th May, 2012. Shigella species was isolated from stool specimens that showed resistant to amoxicillin, nalidixic acid, chloramphenicol and sulfamethoxazole. Water specimen collected from source, distribution reservoir and tap water at school were found grossly contaminated.

Conclusion: The outbreak was caused by novel strain of Shigella species which was not detected earlier in Bhutan. The promotion and provision of boiled water will greatly reduce the incidence of shigellosis especially in boarding facility.

https://doi.org/10.36685/phi.v2i4.98
PDF

References

Farshad S, Sheikhi R, Japoni A, Basiri E, Alborzi A. Characterization of Shigella strains in Iran by plasmid profile analysis and PCR amplification of ipa genes. J Clin Microbiol. 2006;44(8):2879-83.

Ji NN, Liu MM, Huang XR, Zhen J, Li SS, Jiang S, et al. Immunocapture UPPCR combined with DGGE for rapid detection of Shigella species. J Appl Microbiol. 2006;100(4):795-9.

Pettengill EA, Pettengill JB, Binet R. Phylogenetic Analyses of Shigella and Enteroinvasive Escherichia coli for the Identification of Molecular Epidemiological Markers: Whole-Genome Comparative Analysis Does Not Support Distinct Genera Designation. Front Microbiol. 2015;6:1573.

Kahsay AG, Muthupandian S. A review on Sero diversity and antimicrobial resistance patterns of Shigella species in Africa, Asia and South America, 2001-2014. BMC Res Notes. 2016;9(1):422.

Aragon TJ, Vugia DJ, Shallow S, Samuel MC, Reingold A, Angulo FJ, et al. Case-control study of shigellosis in San Francisco: the role of sexual transmission and HIV infection. Clin Infect Dis. 2007;44(3):327-34.

Anderson M, Sansonetti PJ, Marteyn BS. Shigella Diversity and Changing Landscape: Insights for the Twenty-First Century. Front. Cell. Infect. Microbiol. 2016;6:45.

Li Y, Liu D, Cao B, Han W, Liu Y, Liu F, et al. Development of a serotype-specific DNA microarray for identification of some Shigella and pathogenic Escherichia coli strains. J Clin Microbiol. 2006;44(12):4376-83.

Ruekit S, Wangchuk S, Dorji T, Tshering KP, Pootong P, Nobthai P, et al. Molecular characterization and PCR-based replicon typing of multidrug resistant Shigella sonnei isolates from an outbreak in Thimphu, Bhutan. BMC Res Notes. 2014;7:95.

Dorji T, Tsheten, Dorji T, Tshering D. Report on the Shigellosis Outbreak following a traditional festivity at the construction site. Thimphu: Royal Center for Disease Control, 2013.

Darnal JB, Nepal HK, Damchu, Wangchuk LZ, Doung-ngern P, Swaddiwudhipong W. An outbreak of shigellosis in a remote village of Mongar District, Bhutan from March to April 2011. OSIR. 2012;5(12):1 - 8.

Clesceri LS, Greenberg AE, Eaton AD. Standard methods for examination of water and wastewater. 20 ed. Washington, DC: American Public Health Association; 1998.

Clinical and laboratory standards institute. Performance standards for antimicrobial suceptibility testing. 26 ed: Wayne, PA; 2016.

von Seidlein L, Kim DR, Ali M, Lee H, Wang X, Thiem VD, et al. A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations, and microbiology. PLoS medicine. 2006;3(9):e353.

Bennish ML, Khan WA, Begum M, Bridges EA, Ahmed S, Saha D, et al. Low risk of hemolytic uremic syndrome after early effective antimicrobial therapy for Shigella dysenteriae type 1 infection in Bangladesh. Clin Infect Dis. 2006;42(3):356-62.

Kansakar P, Malla S, Ghimire GR. Shigella isolates of Nepal: changes in the incidence of shigella subgroups and trends of antimicrobial susceptibility pattern. Kathmandu Univ Med J. 2007;5(1):32-7.

Khan S, Singh P, Asthana A, Ansari M. Magnitude of drug resistant shigellosis in Nepalese patients. Iran J Microbiol. 2013;5(4):334-8.

Naik DG. Prevalence and antimicrobial susceptibility patterns of Shigella species in Asmara, Eritrea, northeast Africa. J Microbiol Immunol Infect. 2006;39(5):392-5.

Nath R, Saikia L, Choudhury G, Sharma D. Drug resistant Shigella flexneri in & around Dibrugarh, north-east India. Indian J Med Res. 2013;137(1):183-6.

Ndlovu N, Tarupiwa A, Mudzori JT. Antimicrobial resistence of Shigella species isolated during 2004 and 2005 from selected sites in Zimbabwe. Cent Afr J Med. 2006;52(9-12):93-7.

Centers for Disease Control and Prevention. Shigella - Shigellosis [Internet]. [cited Accessed on October 2016]. Available from: http://www.cdc.gov/shigella/general-information.html.

Authors who publish with Public Health of Indonesia agree to the following terms:

  1. Authors retain copyright licensed under a Creative Commons Attribution-NonCommercial CC BY-NC 4.0, which allows others to remix, tweak, and build upon the authors' work non-commercially, and although the others' new works must also acknowledge the authors and be non-commercial, they don't have to license their derivative works on the same terms.
  2. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access). Authors can archive pre-print and post-print or publisher's version/PDF.

Downloads

Download data is not yet available.