Keywords
Extract ethanol Moringa oleifera
Hemoglobin levels
Wistar rats
How to Cite
Abstract
Background: Central Sulawesi falls within the Wallacea region, making it home to various endemic flora and fauna. One distinctive plant in Central Sulawesi is the Moringa tree. The Moringa tree, especially its leaves, was often used as a culinary ingredient known as sayurkelor in the traditional cuisine of the Kaili ethnic group in Central Sulawesi Province.
Objective: This research aimed to determine the effectiveness of moringa leaf extract in increasing hemoglobin levels and erythrocyte count.
Method: Twenty-four male Wistar rats strain (Rattusnorvegicus) 9−10 weeks old and 200−250 grams were divided into four groups (n=6), normal as a no treatment, negative control, ethanol extract, and positive control during the 15thday. Blood samples on the 15thday were administered to determine blood count.
Result: The resultsof this study showed differences in hemoglobin and erythrocyte levels in the four experimental groups.The significant difference in average hemoglobin levels between Group 2 and Group 3 with a P-value <0.05 (P value 0.00). The difference between Group 2 and Group 4 is significant with a P-value <0.05 (P value 0.00), and the difference between Group 3 and Group 4 is significant with a P-value <0.05 (P value 0.03). the significant difference in the average erythrocyte counts between Group 2 and Group 3 with a P-value <0.05 (P value 0.00). The difference between Group 2 and Group 4 is not significant with a P-value >0.05 (P value 0.07). The difference between Group 3 and Group 4 has a P-value >0.05 (P value 0.63).
Conclusion: The research results indicate that pregnant Wistar rats experienced hemodilution characterized by a decrease in hemoglobin and erythrocyte levels during pregnancy. However, after being given ethanol extract from moringa leaves, there was an increase in hemoglobin and erythrocyte levels.
Keywords: Extract; ethanol; moringa; oleifera; hemoglobin
References
Ataide, R., Fielding, K., Pasricha, S., & Bennett, C. (2023). Iron deficiency, pregnancy, and neonatal development. International Journal of Gynecology & Obstetrics, 162(S2), 14–22. https://doi.org/10.1002/ijgo.14944
Auwal, M. M., Yelwa, M. J., Abubakar, I., Jamilu, B., Anchau, G. H., & Tanimu, B. F. (2019). Oriental Journal of Physical Sciences The Levels of Antinutritional Factors in Moringa Oleifera and Vernomia Amygdalina Leaves Found in Some Part of Plateau State , Nigeria. 04(2), 6–10. https://doi.org/http://dx.doi.org/10.13005/OJPS04.02.06
Benson, C. S., Shah, A., Stanworth, S. J., Frise, C. J., Spiby, H., Lax, S. J., Murray, J., & Klein, A. A. (2021). The effect of iron deficiency and anaemia on women’s health. Anaesthesia, 76(S4), 84–95. https://doi.org/10.1111/anae.15405
Camaschella, C. (2019). Iron deficiency. Blood, 133(1), 30–39. https://doi.org/10.1182/blood-2018-05-815944
Cappellini, M. D., Musallam, K. M., & Taher, A. T. (2020). Iron deficiency anaemia revisited. Journal of Internal Medicine, 287(2), 153–170. https://doi.org/10.1111/joim.13004
D’Alessandro, A., Anastasiadi, A. T., Tzounakas, V. L., Nemkov, T., Reisz, J. A., Kriebardis, A. G., Zimring, J. C., Spitalnik, S. L., & Busch, M. P. (2023). Red Blood Cell Metabolism In Vivo and In Vitro. Metabolites, 13(7). https://doi.org/10.3390/metabo13070793
da Silva Lopes, K., Yamaji, N., Rahman, M. O., Suto, M., Takemoto, Y., Garcia-Casal, M. N., & Ota, E. (2021). Nutrition-specific interventions for preventing and controlling anaemia throughout the life cycle: an overview of systematic reviews. The Cochrane Database of Systematic Reviews, 9(9), CD013092. https://doi.org/10.1002/14651858.CD013092.pub2
Derbo, Z. D., & Debelew, G. T. (2023). The Effect of Fresh Moringa Leaf Consumption During Pregnancy on Maternal Hemoglobin Level in Southern Ethiopia: Multilevel Analysis of a Comparative Cross-Sectional Study. International Journal of Women’s Health, 15, 1125–1137. https://doi.org/10.2147/IJWH.S412241
Detlefs, S. E., Jochum, M. D., Salmanian, B., McKinney, J. R., & Aagaard, K. M. (2022). The impact of response to iron therapy on maternal and neonatal outcomes among pregnant women with anemia. American Journal of Obstetrics & Gynecology MFM, 4(2), 100569. https://doi.org/10.1016/j.ajogmf.2022.100569
Devisetti, R., Sreerama, Y. N., & Bhattacharya, S. (2016). Processing effects on bioactive components and functional properties of moringa leaves : development of a snack and quality evaluation. 53(January), 649–657. https://doi.org/10.1007/s13197-015-1962-5
Dinas Kesehatan Provinsi Sulawesi Tengah. (2021). Profil Kesehatan Provinsi Sulawesi Tengah. Profil Kesehatan Provinsi Sulawesi Tengah, 1–377.
Ems, T., Lucia, K. S., & Huecker, M. R. (2023). Biochemistry, Iron Absorption. StatPearles. https://www.ncbi.nlm.nih.gov/books/NBK448204/
Farid, Y., Bowman, N. S., & Lecat, P. (2023). Biochemistry, Hemoglobin Synthesis. In StatPearls. http://www.ncbi.nlm.nih.gov/pubmed/0
Gao, G., Li, J., Zhang, Y., & Chang, Y.-Z. (2019). Cellular Iron Metabolism and Regulation. Advances in Experimental Medicine and Biology, 1173, 21–32. https://doi.org/10.1007/978-981-13-9589-5_2
Garzon, S., Cacciato, P. M., Certelli, C., Salvaggio, C., Magliarditi, M., & Rizzo, G. (2020). Iron deficiency anemia in pregnancy: Novel approaches for an old problem. Oman Medical Journal, 35(5), 1–9. https://doi.org/10.5001/omj.2020.108
Georgieff, M. K. (2020). Iron deficiency in pregnancy. American Journal of Obstetrics and Gynecology, 223(4), 516–524. https://doi.org/10.1016/j.ajog.2020.03.006
Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016). Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5(2), 49–56. https://doi.org/10.1016/j.fshw.2016.04.001
Guo, Y., Zhang, N., Zhang, D., Ren, Q., Ganz, T., Liu, S., & Nemeth, E. (2019). Iron homeostasis in pregnancy and spontaneous abortion. American Journal of Hematology, 94(2), 184–188. https://doi.org/10.1002/ajh.25341
Kashyap, P., Kumar, S., Riar, C. S., Jindal, N., & Baniwal, P. (2022). Recent Advances in Drumstick ( Moringa oleifera ) Leaves Bioactive Compounds : Composition , Health Benefits , Bioaccessibility , and Dietary Applications. 1–37. https://doi.org/10.3390/antiox11020402
Kementerian Kesehatan Republik Indonesia. (2014). Peraturan Menteri Kesehatan Republik Indonesia Nomor 88 Tahun 2014 tentang Standar Tablet Tambah Darah bagi Wanita Usia Subur dan Ibu Hamil. 96, 1–26. https://peraturan.go.id/id/permenkes-no-88-tahun-2014
Khanam, M., Sanin, K. I., Ara, G., Sultana Rita, R., Boitchi, A. B., Farzana, F. D., Haque, M. A., & Ahmed, T. (2022). Effects of Moringa oleifera leaves on hemoglobin and serum retinol levels and underweight status among adolescent girls in rural Bangladesh. Frontiers in Nutrition, 9, 959890. https://doi.org/10.3389/fnut.2022.959890
König, P., Jimenez, K., Saletu-Zyhlarz, G., Mittlböck, M., & Gasche, C. (2020). Iron deficiency, depression, and fatigue in inflammatory bowel diseases. Zeitschrift Für Gastroenterologie, 58(12), 1191–1200. https://doi.org/10.1055/a-1283-6832
Krisnanda, R. (2019). Vitamin C Helps in the Absorption of Iron in Iron Deficiency Anemia. Jurnal Penelitian Perawat Profesional, 2(3). https://doi.org/10.37287/jppp.v2i3.137
Lee, J. Y., Kim, S. E., Park, S. J., Park, M. I., Moon, W., Kim, J. H., & Jung, K. (2022). Helicobacter pylori infection and iron deficiency in non-elderly adults participating in a health check-up program. The Korean Journal of Internal Medicine, 37(2), 304–312. https://doi.org/10.3904/kjim.2020.433
Li, N., Zhao, G., Wu, W., Zhang, M., Liu, W., Chen, Q., & Wang, X. (2020). The Efficacy and Safety of Vitamin C for Iron Supplementation in Adult Patients With Iron Deficiency Anemia. JAMA Network Open, 3(11), e2023644. https://doi.org/10.1001/jamanetworkopen.2020.23644
Matic, I., Guidi, A., Kenzo, M., Mattei, M., & Galgani, A. (2018). Investigation of medicinal plants traditionally used as dietary supplements: A review on Moringa oleifera. Journal of Public Health in Africa, 9(3), 841. https://doi.org/10.4081/jphia.2018.841
Mawouma, S., Ponka, R., & Mbofung, C. M. (2016). Acceptability and solubility of iron and zinc contents of modified Moringa oleifera sauces consumed in the Far- north region of Cameroon. https://doi.org/10.1002/fsn3.398
Meireles, D., Gomes, J., Lopes, L., Hinzmann, M., & Machado, J. (2020). A review of properties, nutritional and pharmaceutical applications of Moringa oleifera: integrative approach on conventional and traditional Asian medicine. Advances in Traditional Medicine, 20(4), 495–515. https://doi.org/10.1007/s13596-020-00468-0
Moustarah, F., & Daley, S. F. (2023). Dietary Iron. In StatPearls. http://www.ncbi.nlm.nih.gov/pubmed/25083899
Mun’im, A., Puteri, M. U., Sari, S. P., & Azizahwati. (2016). Anti-anemia effect of standardized extract of moringa oleifera lamk. Leaves on aniline induced rats. Pharmacognosy Journal, 8(3), 255–258. https://doi.org/10.5530/pj.2016.3.14
Nurhayati, T., Fathoni, M. I., Fatimah, S. N., Tarawan, V. M., Goenawan, H., & Dwiwina, R. G. (2023). Effect of Moringa oleifera Leaf Powder on Hematological Profile of Male Wistar Rats. Journal of Blood Medicine, 14(August), 477–485. https://doi.org/10.2147/JBM.S407884
Pandrangi, S. L., Chittineedi, P., Chalumuri, S. S., Meena, A. S., Neira Mosquera, J. A., Sánchez Llaguno, S. N., Pamuru, R. R., Mohiddin, G. J., & Mohammad, A. (2022). Role of Intracellular Iron in Switching Apoptosis to Ferroptosis to Target Therapy-Resistant Cancer Stem Cells. Molecules (Basel, Switzerland), 27(9). https://doi.org/10.3390/molecules27093011
Pareek, A., Pant, M., Gupta, M. M., Kashania, P., & Ratan, Y. (2023). Moringa oleifera : An Updated Comprehensive Review of Its Pharmacological Activities , Ethnomedicinal , Phytopharmaceutical Formulation , Clinical , Phytochemical , and Toxicological Aspects. https://doi.org/10.3390/ijms24032098.
Pareek, A., Pant, M., Gupta, M. M., Kashania, P., Ratan, Y., Jain, V., Pareek, A., & Chuturgoon, A. A. (2023). Moringa oleifera: An Updated Comprehensive Review of Its Pharmacological Activities, Ethnomedicinal, Phytopharmaceutical Formulation, Clinical, Phytochemical, and Toxicological Aspects. International Journal of Molecular Sciences, 24(3). https://doi.org/10.3390/ijms24032098
Petraglia, F., & Dolmans, M. M. (2022). Iron deficiency anemia: Impact on women’s reproductive health. Fertility and Sterility, 118(4), 605–606. https://doi.org/10.1016/j.fertnstert.2022.08.850
Piskin, E., Cianciosi, D., Gulec, S., Tomas, M., & Capanoglu, E. (2022). Iron Absorption: Factors, Limitations, and Improvement Methods. ACS Omega, 7(24), 20441–20456. https://doi.org/10.1021/acsomega.2c01833
Pitopang, R., Moh.Ihsan, & Burhanuddin, I. F. (2014). Panduan Pengenalan Flora dan Fauna Endemik. https://www.researchgate.net/profile/Ramadanil-Pitopang/publication/327426752_anduan_Pengenalan_Flora_Fauna_Endemik_Kabupaten_Sigi_Sulawesi_Tengah/links/5b8ecbd692851c6b7ebfb109/anduan-Pengenalan-Flora-Fauna-Endemik-Kabupaten-Sigi-Sulawesi-Tengah.pdf
Prayugi, S. D., Ansar, & Lewa, A. F. (2015). Faktor Sosial Budaya Yang Berhubungan Dengan Pola Konsumsi Makanan Pada Masyarakat Suku Kaili Di Kota Palu Propinsi Sulawesi Tengah. Jurnal Ilmu Kesehatan, 1(18), 927–934. https://docplayer.info/53430909-Faktor-sosial-budaya-yang-berhubungan-dengan-pola-konsumsi-makanan-pada-masyarakat-suku-kaili-di-kota-palu-propinsi-sulawesi-tengah.html
Rotella, R., Soriano, J. M., Llopis-Gonzalez, A., & Morales-Suarez-Varela, M. (2023). The Impact of Moringa oleifera Supplementation on Anemia and other Variables during Pregnancy and Breastfeeding : A Narrative Review. https://doi.org/10.3390/nu15122674
Saleh, S. (2013). Kearifan Lokal Masyarakat Kaili di Sulawesi Tengah. Academica Fisip Untad, 05(02), 1126–1134. https://www.neliti.com/id/publications/28500/kearifan-lokal-masyarakat-kaili-di-sulawesi-tengah
Segwatibe, M. K., Cosa, S., & Bassey, K. (2023). Antioxidant and Antimicrobial Evaluations of Moringa oleifera Lam Leaves Extract and Isolated Compounds. Molecules (Basel, Switzerland), 28(2). https://doi.org/10.3390/molecules28020899
Stoffel, N. U., von Siebenthal, H. K., Moretti, D., & Zimmermann, M. B. (2020). Oral iron supplementation in iron-deficient women: How much and how often? Molecular Aspects of Medicine, 75, 100865. https://doi.org/10.1016/j.mam.2020.100865
Su, X., Lu, G., Ye, L., Shi, R., Zhu, M., Yu, X., Li, Z., Jia, X., & Feng, L. (2023). active components , health bene fi ts and. 24353–24384. https://doi.org/10.1039/d3ra03584k
Sultana, S. (2020). Nutritional and functional properties of Moringa oleifera. Metabolism Open, 8, 100061. https://doi.org/10.1016/j.metop.2020.100061
Suzana, D., Suyatna, F. D., A, A., Andrajati, R., Sari, S. P., & Mun’im, A. (2017). Effect of Moringa oleifera Leaves Extract Against Hematology and Blood Biochemical Value of Patients with Iron Deficiency Anemia. Journal of Young Pharmacists, 9(1s), s79–s84. https://doi.org/10.5530/jyp.2017.1s.20
Wardana, M. S., Jufri, M., & Mun’im, A. (2022). Physicochemical Properties and Nutrition of Moringa Oleifera Lam. Leaf extract: a prelimenary study on Preparation Phytosomes as Herbal Supplements for Children. International Journal of Applied Pharmaceutics, 281–287. https://doi.org/10.22159/ijap.2022v14i1.43477
Xiao, X., Wang, J., Meng, C., Liang, W., Wang, T., Zhou, B., Wang, Y., Luo, X., Gao, L., & Zhang, L. (2020). Moringa oleifera Lam and its Therapeutic Effects in Immune Disorders. Frontiers in Pharmacology, 11, 566783. https://doi.org/10.3389/fphar.2020.566783
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